Hotspot

rps15-ycf1

SSC · 122544-122940 · 397 bp

Back to Meconopsis

Pi 0.0448
MarkerSeek score 79.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
397
variable_sites
71
pi
0.0448
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GCGGAATAACTGAGATGAATGC ACCAGTAATGGAAGTCGTTTCA 45.5 40.9 57.9 57.7 478-498 bp 0.8 48.1
rps15-ycf1_p2 GCGGAATAACTGAGATGAATGC TGGAAGTCGTTTCAGTATGTCA 45.5 40.9 57.9 57.4 470-490 bp 0.8 46.1
rps15-ycf1_p3 GCGGAATAACTGAGATGAATGC CCAGTAATGGAAGTCGTTTCAG 45.5 45.5 57.9 57.1 477-497 bp 0.8 44.0
rps15-ycf1_p4 GCGGAATAACTGAGATGAATGC AGTCGTTTCAGTATGTCAAGGA 45.5 40.9 57.9 57.1 466-486 bp 0.733333 41.3
rps15-ycf1_p5 TCTTGCGGAATAACTGAGATGA ACCAGTAATGGAAGTCGTTTCA 40.9 40.9 57.3 57.7 482-502 bp 0.733333 41.3