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psaC-ndhE

SSC · 116670-116931 · 262 bp

Back to Meconopsis

Pi 0.0528

MarkerSeek score 83.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 262
variable_sites: 53
pi: 0.0528
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAGGCTCGTACACATTGAGT	TATTGGGCCAGCCATTGTTT	50.0	45.0	57.6	58.1	398-415 bp	1.0	88.6
psaC-ndhE_p2	CAGGCTCGTACACATTGAGT	CTATTGGGCCAGCCATTGTT	50.0	50.0	57.6	58.5	399-416 bp	1.0	84.9
psaC-ndhE_p3	GCAGGCTCGTACACATTGAG	TATTGGGCCAGCCATTGTTT	55.0	45.0	59.0	58.1	399-416 bp	1.0	83.6
psaC-ndhE_p4	GCAGGCTCGTACACATTGA	TATTGGGCCAGCCATTGTTT	52.6	45.0	57.9	58.1	399-416 bp	1.0	82.5
psaC-ndhE_p5	CAGGCTCGTACACATTGAGT	CCAGCCATTGTTTCGTCAAT	50.0	45.0	57.6	57.0	391-408 bp	1.0	80.9