Hotspot

ccsA-ndhD

SSC · 114543-114792 · 250 bp

Back to Meconopsis

Pi 0.0689
MarkerSeek score 82.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
250
variable_sites
60
pi
0.0689
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGGTATGCTATTTTGGGGTCA TCAGTTGACAAGGTAGAAGCT 47.6 42.9 57.7 56.6 355-393 bp 1.0 61.8
ccsA-ndhD_p2 TGGGTATGCTATTTTGGGGTC TCAGTTGACAAGGTAGAAGCT 47.6 42.9 57.7 56.6 356-394 bp 1.0 61.8
ccsA-ndhD_p3 TGGGTATGCTATTTTGGGGT TCAGTTGACAAGGTAGAAGCT 45.0 42.9 56.5 56.6 356-394 bp 1.0 60.0
ccsA-ndhD_p4 TTGGGTATGCTATTTTGGGGT TCAGTTGACAAGGTAGAAGCT 42.9 42.9 57.2 56.6 357-395 bp 1.0 58.1
ccsA-ndhD_p5 GGGTATGCTATTTTGGGGTCA ATCAGTTGACAAGGTAGAAGCT 47.6 40.9 57.7 57.1 356-394 bp 1.0 57.9