Hotspot

petA-psbJ

LSC · 62554-63626 · 1073 bp

Back to Meconopsis

Pi 0.0375

MarkerSeek score 83.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 148
pi: 0.0375
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCGAGAAGGTTCAATTGTCTGA	ACTGTAGCCGGTATTCCTGT	40.9	50.0	57.4	58.1	1139-1237 bp	0.933333	67.4
petA-psbJ_p2	TCGAGAAGGTTCAATTGTCTGA	TGTAGCCGGTATTCCTGTGA	40.9	50.0	57.4	58.1	1137-1235 bp	0.933333	67.4
petA-psbJ_p3	TCGAGAAGGTTCAATTGTCTGA	CTGTAGCCGGTATTCCTGTG	40.9	55.0	57.4	57.8	1138-1236 bp	0.933333	66.7
petA-psbJ_p4	TCGAGAAGGTTCAATTGTCTGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.4	57.6	1084-1182 bp	0.933333	65.6
petA-psbJ_p5	TCGAGAAGGTTCAATTGTCTGAA	ACTGTAGCCGGTATTCCTGT	39.1	50.0	58.0	58.1	1139-1237 bp	0.933333	63.9