Hotspot

accD-psaI

LSC · 58663-59129 · 467 bp

Back to Meconopsis

Pi 0.0439

MarkerSeek score 80.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 73
pi: 0.0439
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATTGCATTTGCGGGTAAACG	GTTCTGAGCGCCAGTTCATA	45.0	50.0	58.0	58.0	954-976 bp	1.0	90.3
accD-psaI_p2	CCGGGTACTTGGGATCCTAT	GTTCTGAGCGCCAGTTCATA	55.0	50.0	58.0	58.0	1434-1456 bp	1.0	88.2
accD-psaI_p3	ATTGCATTTGCGGGTAAACG	TTCTGAGCGCCAGTTCATAC	45.0	50.0	58.0	58.0	955-975 bp	0.933333	87.1
accD-psaI_p4	CCGGGTACTTGGGATCCTAT	TTCTGAGCGCCAGTTCATAC	55.0	50.0	58.0	58.0	1435-1455 bp	0.933333	84.5
accD-psaI_p5	TTGCCGAACCTAATGCCTAC	TTCTGAGCGCCAGTTCATAC	50.0	50.0	58.0	58.0	975-995 bp	0.733333	78.4