Hotspot

ndhC-trnV-UAC

LSC · 49958-51406 · 1449 bp

Back to Meconopsis

Pi 0.033
MarkerSeek score 80.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1449
variable_sites
196
pi
0.033
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAAATGCCCAGAAAATGTCA AGAAGGTCTACGGTTCGAGT 38.1 50.0 56.0 57.8 1432-1573 bp 1.0 66.6
ndhC-trnV-UAC_p2 TGCCCAGAAAATGTCATATTCG AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.1 57.8 1427-1568 bp 0.933333 64.6
ndhC-trnV-UAC_p3 GCCCAGAAAATGTCATATTCGT AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.1 57.8 1426-1567 bp 0.933333 64.5
ndhC-trnV-UAC_p4 TGCCCAGAAAATGTCATATTCG GAAGGTCTACGGTTCGAGTC 40.9 55.0 57.1 57.5 1426-1567 bp 0.933333 61.8
ndhC-trnV-UAC_p5 GCCCAGAAAATGTCATATTCGT GAAGGTCTACGGTTCGAGTC 40.9 55.0 57.1 57.5 1425-1566 bp 0.933333 61.8