Hotspot

trnL-UAA-trnF-GAA

LSC · 47097-47456 · 360 bp

Back to Meconopsis

Pi 0.038

MarkerSeek score 80.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 360
variable_sites: 58
pi: 0.038
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	440-450 bp	1.0	87.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	437-447 bp	1.0	84.5
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	429-439 bp	1.0	83.9
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAA	50.0	50.0	57.5	57.4	416-426 bp	1.0	83.4
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	412-422 bp	1.0	83.4