Hotspot

psbM-trnD-GUC

LSC · 29086-30213 · 1128 bp

Back to Meconopsis

Pi 0.0301

MarkerSeek score 81.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 132
pi: 0.0301
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.3	58.5	914-1247 bp	1.0	86.1
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.6	58.5	913-1246 bp	1.0	85.2
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.5	58.5	915-1248 bp	1.0	84.4
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.6	58.5	917-1250 bp	1.0	83.1
psbM-trnD-GUC_p5	ACTAGAATGAACAGCGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	920-1253 bp	1.0	82.2