Hotspot

rpoB-trnC-GCA

LSC · 26696-27656 · 961 bp

Back to Meconopsis

Pi 0.0315

MarkerSeek score 81.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 118
pi: 0.0315
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATGAACCTGCAAAACCCCT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.9	58.4	1020-1143 bp	1.0	88.3
rpoB-trnC-GCA_p2	ACCTGCAAAACCCCTCAAAT	AAAAGGATTTGCAGTCCCCC	45.0	50.0	57.9	58.4	1015-1138 bp	1.0	88.3
rpoB-trnC-GCA_p3	AACCTGCAAAACCCCTCAAA	AAAAGGATTTGCAGTCCCCC	45.0	50.0	58.1	58.4	1016-1139 bp	1.0	88.1
rpoB-trnC-GCA_p4	CAATGAACCTGCAAAACCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	1021-1144 bp	1.0	87.8
rpoB-trnC-GCA_p5	CCCTGATCAATGAACCTGCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1094 bp	0.066667	38.8