Hotspot

rps16-trnQ-UUG

LSC · 5895-6801 · 907 bp

Back to Meconopsis

Pi 0.0417
MarkerSeek score 85.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
907
variable_sites
152
pi
0.0417
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 980-1205 bp 1.0 90.3
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 991-1216 bp 1.0 89.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 984-1209 bp 1.0 89.2
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 995-1220 bp 1.0 88.6
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA ACTCGGAGGTTCGAATCCTT 45.0 50.0 58.1 58.4 985-1210 bp 1.0 87.3