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trnH-GUG-psbA

LSC · 116-354 · 239 bp

Back to Meconopsis

Pi 0.0697

MarkerSeek score 81.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 62
pi: 0.0697
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	305-386 bp	0.933333	87.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	319-400 bp	0.933333	87.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	318-399 bp	0.933333	87.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	320-401 bp	0.933333	87.7
trnH-GUG-psbA_p5	GCCTTGATCCACTTGGCTAC	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.6	57.9	310-391 bp	0.933333	83.8