Hotspot

ccdA

Genome · 107310-108050 · 741 bp

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Pi 0.085

MarkerSeek score 77.30

Primer available yes

Label ccdA

Feature Metadata

label_name: ccdA
feature_type: gene
region: Genome
length_bp: 741
variable_sites: 63
pi: 0.085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccdA_p1	AGCGTGTCTACCAATTCCAC	AAGCCTGGTAATTGACGAGA	50.0	45.0	57.9	56.6	1408-1414 bp	1.0	73.9
ccdA_p2	AGCGTGTCTACCAATTCCAC	AGCCTGGTAATTGACGAGAA	50.0	45.0	57.9	56.6	1407-1413 bp	1.0	73.9
ccdA_p3	GGTTCAAATCCTTCCTGGGG	AAGCCTGGTAATTGACGAGA	55.0	45.0	58.1	56.6	1160 bp	1.0	73.0
ccdA_p4	GGTTCAAATCCTTCCTGGGG	AGCCTGGTAATTGACGAGAA	55.0	45.0	58.1	56.6	1159 bp	1.0	73.0
ccdA_p5	AAATCCTTCCTGGGGAGGTT	AGCCTGGTAATTGACGAGAA	50.0	45.0	58.2	56.6	1154 bp	1.0	72.4