Hotspot

clpC-rpl19

Genome · 141856-142854 · 999 bp

Back to Mazzaella

Pi 0.125

MarkerSeek score 80.80

Primer available yes

Label clpC-rpl19

Feature Metadata

label_name: clpC-rpl19
feature_type: igs
region: Genome
length_bp: 999
variable_sites: 125
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-rpl19_p1	TTTCTACCGCAACAAACCCA	GGCAAAACCAGCGGATTTAC	45.0	50.0	57.9	58.0	1861-1866 bp	1.0	86.9
clpC-rpl19_p2	TTTCTACCGCAACAAACCCA	AGGCAAAACCAGCGGATTTA	45.0	45.0	57.9	58.1	1862-1867 bp	1.0	86.3
clpC-rpl19_p3	TTTCTACCGCAACAAACCCA	TAGGCAAAACCAGCGGATTT	45.0	45.0	57.9	58.1	1863-1868 bp	1.0	86.3
clpC-rpl19_p4	TTTCTACCGCAACAAACCCA	AACCAGCGGATTTACAGTCC	45.0	50.0	57.9	57.9	1856-1861 bp	1.0	86.1
clpC-rpl19_p5	GTGCAGCAATACCTGTTCCT	GGCAAAACCAGCGGATTTAC	50.0	50.0	58.2	58.0	1768-1773 bp	1.0	86.0