Hotspot

trnQ(ttg)-psb28

Genome · 126500-126778 · 279 bp

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Pi 0.0993
MarkerSeek score 77.80
Primer available yes
Label trnQ(ttg)-psb28

Feature Metadata

label_name
trnQ(ttg)-psb28
feature_type
igs
region
Genome
length_bp
279
variable_sites
27
pi
0.0993
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ(ttg)-psb28_p1 TTTGACTCCGCCATTCGTAG CCTTTAATGAATTGAATACTCGCCA 50.0 36.0 58.0 58.0 349-534 bp 1.0 46.2
trnQ(ttg)-psb28_p2 TTTGACTCCGCCATTCGTAG AATGAATTGAATACTCGCCA 50.0 35.0 58.0 53.0 344-529 bp 1.0 46.2
trnQ(ttg)-psb28_p3 CTTTGACTCCGCCATTCGTA CCTTTAATGAATTGAATACTCGCCA 50.0 36.0 58.0 58.0 350-535 bp 1.0 46.1
trnQ(ttg)-psb28_p4 CTTTGACTCCGCCATTCGTA ACCTTTAATGAATTGAATACTCGCC 50.0 36.0 58.0 58.0 351-536 bp 1.0 46.1
trnQ(ttg)-psb28_p5 TTTGACTCCGCCATTCGTAG ACCTTTAATGAATTGAATACTCGCC 50.0 36.0 58.0 58.0 350-535 bp 1.0 46.1