Hotspot

ycf27-psbD

Genome · 117931-118377 · 447 bp

Back to Mazzaella

Pi 0.0952

MarkerSeek score 80.10

Primer available yes

Label ycf27-psbD

Feature Metadata

label_name: ycf27-psbD
feature_type: igs
region: Genome
length_bp: 447
variable_sites: 40
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf27-psbD_p1	TGATGCCCAAGTTAGATGGC	GATTCCTACAAGCCGAGCAA	50.0	50.0	57.9	58.0	1415 bp	0.5	59.3
ycf27-psbD_p2	AACTCACTGTATCCGCACAC	GATTCCTACAAGCCGAGCAA	50.0	50.0	57.9	58.0	1214 bp	0.5	59.2
ycf27-psbD_p3	TGATGCCCAAGTTAGATGGC	AGCGCATAAAAGAGCTCCTC	50.0	50.0	57.9	58.1	1637 bp	0.5	59.1
ycf27-psbD_p4	TGATGCCCAAGTTAGATGGC	GGTCTGATTCCTACAAGCCG	50.0	55.0	57.9	58.1	1420 bp	0.5	59.1
ycf27-psbD_p5	AACTCACTGTATCCGCACAC	AGCGCATAAAAGAGCTCCTC	50.0	50.0	57.9	58.1	1436 bp	0.5	58.9