Hotspot

trnP(tgg)-ycf52

Genome · 90703-90757 · 55 bp

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Pi 0.164
MarkerSeek score 78.80
Primer available yes
Label trnP(tgg)-ycf52

Feature Metadata

label_name
trnP(tgg)-ycf52
feature_type
igs
region
Genome
length_bp
55
variable_sites
9
pi
0.164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP(tgg)-ycf52_p1 GCTACCAAGCTGCGCTATAT AGGTATGTTTTGGTATCCACGT 50.0 40.9 57.9 57.4 104-106 bp 1.0 65.7
trnP(tgg)-ycf52_p2 GCTACCAAGCTGCGCTATAT TGTTTTGGTATCCACGTTGA 50.0 40.0 57.9 55.2 99-101 bp 1.0 64.2
trnP(tgg)-ycf52_p3 TTTGAACCTGCGACATCCTG AGGTATGTTTTGGTATCCACGT 50.0 40.9 58.5 57.4 140-142 bp 1.0 60.8
trnP(tgg)-ycf52_p4 CAGGATTTGAACCTGCGACA AGGTATGTTTTGGTATCCACGT 50.0 40.9 58.5 57.4 145-147 bp 1.0 60.6
trnP(tgg)-ycf52_p5 ATTTGAACCTGCGACATCCT AGGTATGTTTTGGTATCCACGT 45.0 40.9 57.5 57.4 141-143 bp 1.0 60.6