Hotspot

psbE-ycf63

Genome · 84932-85643 · 712 bp

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Pi 0.0994

MarkerSeek score 79.90

Primer available yes

Label psbE-ycf63

Feature Metadata

label_name: psbE-ycf63
feature_type: igs
region: Genome
length_bp: 712
variable_sites: 70
pi: 0.0994
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-ycf63_p1	ACCCTGCTACAAAAAGTGCA	GCAAGAGGAGTCGGTCAATC	45.0	55.0	57.9	58.4	944-973 bp	1.0	84.3
psbE-ycf63_p2	ACCCTGCTACAAAAAGTGCA	AGCTGTTCTTACATTGGCGT	45.0	45.0	57.9	57.8	1359 bp	0.5	58.0
psbE-ycf63_p3	TGGCTGCTATACCACCTACA	AGCTGTTCTTACATTGGCGT	50.0	45.0	57.8	57.8	1937 bp	0.5	57.2
psbE-ycf63_p4	ACCCTGCTACAAAAAGTGCA	TTGGTCGTGTAGGATCATGC	45.0	50.0	57.9	57.7	1297 bp	0.5	57.0
psbE-ycf63_p5	AAAAAGGACGTTCACCCGTT	AGCTGTTCTTACATTGGCGT	45.0	45.0	58.2	57.8	1287 bp	0.5	56.6