Hotspot

cbbX-trnC(gca)

Genome · 55311-55400 · 90 bp

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Pi 0.113

MarkerSeek score 81.00

Primer available yes

Label cbbX-trnC(gca)

Feature Metadata

label_name: cbbX-trnC(gca)
feature_type: igs
region: Genome
length_bp: 90
variable_sites: 9
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cbbX-trnC(gca)_p1	ACGATTTAGTAACTATACAATCAGA	TCTGCCTTACCACTTGGCTA	28.0	50.0	53.7	58.3	161-176 bp	1.0	48.3
cbbX-trnC(gca)_p2	ACGATTTAGTAACTATACAATCAGA	TTGCAATCCTCTGCCTTACC	28.0	50.0	53.7	57.9	170-185 bp	1.0	48.1
cbbX-trnC(gca)_p3	ACGATTTAGTAACTATACAATCAGA	GGATTTGCAATCCTCTGCCT	28.0	50.0	53.7	58.2	174-189 bp	1.0	48.0
cbbX-trnC(gca)_p4	ACGATTTAGTAACTATACAATCAGA	AGGATTTGCAATCCTCTGCC	28.0	50.0	53.7	58.2	175-190 bp	1.0	47.9
cbbX-trnC(gca)_p5	ACGATTTAGTAACTATACAATCAGA	TTTTAGGCGGTACCCAGACT	28.0	50.0	53.7	58.4	209-224 bp	1.0	47.3