Hotspot

pbsA-rpl35

Genome · 6057-6764 · 708 bp

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Pi 0.0919

MarkerSeek score 79.40

Primer available yes

Label pbsA-rpl35

Feature Metadata

label_name: pbsA-rpl35
feature_type: igs
region: Genome
length_bp: 708
variable_sites: 65
pi: 0.0919
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pbsA-rpl35_p1	GAACCGAGATTCTGGTGGTC	AGACTTGCTGCTTTGCTGTA	55.0	45.0	58.0	57.7	1692-1728 bp	1.0	83.7
pbsA-rpl35_p2	GAACCGAGATTCTGGTGGTC	GCAACAAATGACTGCGTGAA	55.0	45.0	58.0	57.9	1388 bp	0.5	59.0
pbsA-rpl35_p3	ATTGGCAACCAGGTAATGCA	GCAACAAATGACTGCGTGAA	45.0	45.0	58.1	57.9	1131 bp	0.5	58.5
pbsA-rpl35_p4	AACGAACCGAGATTCTGGTG	GCAACAAATGACTGCGTGAA	50.0	45.0	57.9	57.9	1391 bp	0.5	58.4
pbsA-rpl35_p5	AACCCGGATGAAGTTTGTCC	GCAACAAATGACTGCGTGAA	50.0	45.0	58.1	57.9	1156 bp	0.5	58.3