Hotspot

ndhF

SSC · 109501-111714 · 2214 bp

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Pi 0.0124
MarkerSeek score 42.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2214
variable_sites
88
pi
0.0124
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TAGCCCCCTTTCTGAAGAGT GCGATACCCCTTCCTTTTCC 50.0 55.0 57.7 58.3 2749-2899 bp 1.0 82.0
ndhF_p2 TAGCCCCCTTTCTGAAGAGT GCTTTTAATGCTGCGCGATA 50.0 45.0 57.7 57.6 2763-2913 bp 1.0 81.5
ndhF_p3 TAGCCCCCTTTCTGAAGAGT AAACCGCTTTTAATGCTGCG 50.0 45.0 57.7 58.3 2888-2918 bp 0.777778 66.8
ndhF_p4 GGAAGAAAAGGCGGATCTGA AAACCGCTTTTAATGCTGCG 50.0 45.0 57.6 58.3 2993-2994 bp 0.555556 56.9
ndhF_p5 GGAAGAAAAGGCGGATCTGA GCGATACCCCTTCCTTTTCC 50.0 55.0 57.6 58.3 2974-2975 bp 0.555556 56.7