Hotspot

ndhF-rpl32

SSC · 111715-112225 · 511 bp

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Pi 0.0188
MarkerSeek score 62.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
511
variable_sites
27
pi
0.0188
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ATCCCCCACACGGTTAGTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1759-2109 bp 1.0 87.1
ndhF-rpl32_p2 ATCCCCCACACGGTTAGTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1760-2110 bp 1.0 87.1
ndhF-rpl32_p3 ACAAACGCCTTTTGACAAGC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1740-2090 bp 1.0 86.8
ndhF-rpl32_p4 CAAACGCCTTTTGACAAGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1739-2089 bp 1.0 86.8
ndhF-rpl32_p5 ACAAACGCCTTTTGACAAGC CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1741-2091 bp 1.0 86.8