Hotspot

rpl20-rps12

LSC · 68745-69570 · 826 bp

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Pi 0.0164
MarkerSeek score 59.90
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
826
variable_sites
42
pi
0.0164
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT CAGCGTCGAGGAACATGTAC 50.0 55.0 58.0 58.7 887-899 bp 1.0 79.8
rpl20-rps12_p2 CAGTCTTGAATGAGCCCCTC CAGCGTCGAGGAACATGTAC 55.0 55.0 58.0 58.7 946-958 bp 1.0 79.4
rpl20-rps12_p3 CTTGAATGAGCCCCTCGAAA CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 942-954 bp 1.0 78.8
rpl20-rps12_p4 GAATGAGCCCCTCGAAAACT CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 939-951 bp 1.0 78.8
rpl20-rps12_p5 GAGCCCCTCGAAAACTTGAT CAGCGTCGAGGAACATGTAC 50.0 55.0 57.9 58.7 935-947 bp 1.0 78.8