Hotspot

ndhC-trnV-UAC

LSC · 49929-51114 · 1186 bp

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Pi 0.0151
MarkerSeek score 56.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1186
variable_sites
58
pi
0.0151
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.3 57.8 1288-1301 bp 1.0 45.1
ndhC-trnV-UAC_p2 GACTTGATATTATTAGAAACGCCCA AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.3 57.8 1271-1284 bp 1.0 45.1
ndhC-trnV-UAC_p3 ACTTGATATTATTAGAAACGCCCA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.2 57.8 1270-1283 bp 1.0 45.1
ndhC-trnV-UAC_p4 AGACTTGATATTATTAGAAACGCCC AGAAGGTCTACGGTTCGAGT 36.0 50.0 57.0 57.8 1272-1285 bp 1.0 45.1
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.3 57.5 1287-1300 bp 1.0 45.1