Hotspot

trnT-UGU-trnL-UAA

LSC · 46040-46652 · 613 bp

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Pi 0.0175
MarkerSeek score 55.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
613
variable_sites
36
pi
0.0175
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG GCTTCCATTGAGTCTCTGCA 55.0 50.0 57.9 57.9 849-891 bp 1.0 85.6
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG GGCTTTTTGGGGATAGAGGG 55.0 55.0 57.9 57.9 1227-1269 bp 1.0 84.6
trnT-UGU-trnL-UAA_p3 CGAAGCCATACCCAATCGAA GCTTCCATTGAGTCTCTGCA 50.0 50.0 58.1 57.9 1521-1577 bp 1.0 84.4
trnT-UGU-trnL-UAA_p4 CGAAGCCATACCCAATCGAA GGCTTTTTGGGGATAGAGGG 50.0 55.0 58.1 57.9 1899-1955 bp 1.0 84.0
trnT-UGU-trnL-UAA_p5 CGAAGCCATACCCAATCGAA AGGATTTGGCTCAGGATTGC 50.0 50.0 58.1 58.2 1450-1505 bp 1.0 83.7