Hotspot

trnG-GCC-trnfM-CAU

LSC · 35864-36028 · 165 bp

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Pi 0.0387
MarkerSeek score 63.20
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
165
variable_sites
17
pi
0.0387
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 217-229 bp 1.0 86.4
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT GTTCAAATCCTGTCTCCGCA 50.0 50.0 58.1 58.2 218-230 bp 1.0 86.4
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 266-278 bp 1.0 86.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 267-279 bp 1.0 86.2
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.1 57.8 250-262 bp 1.0 85.0