Hotspot

ycf1

SSC · 127152-132917 · 5766 bp

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Pi 0.00301

MarkerSeek score 43.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5766
variable_sites: 26
pi: 0.00301
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTCATTCATATAACCCCAACGG	AAAGAAAACCCAACGTTCGA	43.5	40.0	57.6	56.1	325 bp	1.0	40.7
ycf1_p2	GTTCATTCATATAACCCCAACGG	AAGAAAACCCAACGTTCGAA	43.5	40.0	57.6	56.1	324 bp	1.0	40.7
ycf1_p3	GTTCATTCATATAACCCCAACGG	AGAAAACCCAACGTTCGAAA	43.5	40.0	57.6	56.1	323 bp	1.0	40.7
ycf1_p4	TCATTCATATAACCCCAACGG	AAGAAAACCCAACGTTCGAA	42.9	40.0	55.4	56.1	322 bp	1.0	40.7
ycf1_p5	TCATTCATATAACCCCAACGG	AGAAAACCCAACGTTCGAAA	42.9	40.0	55.4	56.1	321 bp	1.0	40.7