Hotspot

rpl22

LSC · 86082-86642 · 561 bp

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Pi 0.00654

MarkerSeek score 53.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 561
variable_sites: 5
pi: 0.00654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1041-1358 bp	1.0	84.0
rpl22_p2	CGCCCTGCAATTTGTACTTG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1532-1849 bp	1.0	83.9
rpl22_p3	GTCCGGCTAGAAATTCTGCA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1432-1749 bp	1.0	83.9
rpl22_p4	TGTCCGGCTAGAAATTCTGC	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1433-1750 bp	1.0	83.9
rpl22_p5	TTGTCCCATAATCCCCCACT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	949-1266 bp	1.0	83.7