Hotspot

rbcL

LSC · 57444-58871 · 1428 bp

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Pi 0.00187

MarkerSeek score 59.90

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 4
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGATTCCAGTGAAAGGCTTCA	TGGGCCGAGTTAAATTGCAA	42.9	45.0	57.2	58.4	1582 bp	1.0	62.8
rbcL_p2	AGATTCCAGTGAAAGGCTTCA	TTGGGCCGAGTTAAATTGCA	42.9	45.0	57.2	58.4	1583 bp	1.0	62.8
rbcL_p3	TCGAGTAGACCTTGTTGTTGT	TGGGCCGAGTTAAATTGCAA	42.9	45.0	56.9	58.4	1544 bp	1.0	60.5
rbcL_p4	TCGAGTAGACCTTGTTGTTGT	TTGGGCCGAGTTAAATTGCA	42.9	45.0	56.9	58.4	1545 bp	1.0	60.5
rbcL_p5	AAGATTCCAGTGAAAGGCTTCA	TGGGCCGAGTTAAATTGCAA	40.9	45.0	57.9	58.4	1583 bp	1.0	60.3