Hotspot

psbC

LSC · 34841-36262 · 1422 bp

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Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.00141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCTCAGGAAATCCGTGCAG	AGCCATGGTGGAAGTAAATGA	50.0	42.9	57.9	57.3	1583 bp	1.0	65.5
psbC_p2	TTCTCAGGAAATCCGTGCAG	TCAAGTAGTGGATCTCTTGCC	50.0	47.6	57.9	57.1	1553 bp	1.0	64.2
psbC_p3	AGGTATTCGTGCTTGGATGG	AGCCATGGTGGAAGTAAATGA	50.0	42.9	57.7	57.3	1511 bp	1.0	63.6
psbC_p4	TTCTCAGGAAATCCGTGCAG	AGCCATGGTGGAAGTAAATGAA	50.0	40.9	57.9	58.0	1583 bp	1.0	63.0
psbC_p5	TTCTCAGGAAATCCGTGCAG	TGACCCAATCTACCCAACTAGA	50.0	45.5	57.9	57.9	1618 bp	1.0	62.8