Hotspot

psbA

LSC · 300-1361 · 1062 bp

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Pi 0.000628

MarkerSeek score 45.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGGTTGCTATTGCTCCTTCA	TGCTTGGGAGTCCCTGATAA	50.0	50.0	57.9	58.0	1167 bp	1.0	78.8
psbA_p2	GGGTTGCTATTGCTCCTTCA	TAGAGAATGCGTGTGCTTGG	50.0	50.0	57.9	58.3	1180 bp	1.0	76.8
psbA_p3	AGGGTTGCTATTGCTCCTTC	TGCTTGGGAGTCCCTGATAA	50.0	50.0	57.6	58.0	1168 bp	1.0	76.4
psbA_p4	TCTCAAAAACCCGAACTCGT	TGCTTGGGAGTCCCTGATAA	45.0	50.0	57.4	58.0	1146 bp	1.0	74.9
psbA_p5	AGGGTTGCTATTGCTCCTTC	TAGAGAATGCGTGTGCTTGG	50.0	50.0	57.6	58.3	1181 bp	1.0	74.5