Hotspot

trnN-GUU-ndhF

IRb · 112053-114182 · 2130 bp

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Pi 0.00438

MarkerSeek score 52.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2130
variable_sites: 14
pi: 0.00438
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	AAAGAGGGGCTAAGTCCGAT	55.0	50.0	58.0	58.1	2254-2255 bp	1.0	79.4
trnN-GUU-ndhF_p2	ACGACCAGTCAGTTAACAGC	AAAGAGGGGCTAAGTCCGAT	50.0	50.0	57.8	58.1	2229-2230 bp	1.0	78.4
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	AAAGAGGGGCTAAGTCCGAT	50.0	50.0	58.2	58.1	2242-2243 bp	1.0	78.3
trnN-GUU-ndhF_p4	ATCTCCCCAGGTAGGATTCG	ATGGGGTAAAGAGGGGCTAA	55.0	50.0	58.0	57.7	2261-2262 bp	1.0	78.0
trnN-GUU-ndhF_p5	ATCTCCCCAGGTAGGATTCG	TTATGGGGTAAAGAGGGGCT	55.0	50.0	58.0	57.7	2263-2264 bp	1.0	78.0