Hotspot

rpl16-rps3

LSC · 85144-85309 · 166 bp

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Pi 0.012

MarkerSeek score 60.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 3
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	AGAGAAGGTAGGGTTCCTCT	45.0	50.0	57.1	56.4	307 bp	1.0	60.6
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	TGCTCAAACTCTTTATGGGGT	45.0	42.9	57.1	57.2	248 bp	1.0	59.4
rpl16-rps3_p3	ACGAGTCACACACTAAGCAT	AGAGAAGGTAGGGTTCCTCT	45.0	50.0	56.9	56.4	306 bp	1.0	58.8
rpl16-rps3_p4	CAACGAGTCACACACTAAGC	AGAGAAGGTAGGGTTCCTCT	50.0	50.0	56.8	56.4	308 bp	1.0	58.0
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	GAGAAGGTAGGGTTCCTCTACA	45.0	50.0	57.1	58.0	306 bp	1.0	57.5