Hotspot

psaI-ycf4

LSC · 61266-61749 · 484 bp

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Pi 0.00551

MarkerSeek score 59.80

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 4
pi: 0.00551
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GGCAATTGCAATGGCTTCTT	CGTTCTGACCGACAACTCAT	45.0	50.0	57.9	57.9	559 bp	1.0	79.2
psaI-ycf4_p2	GTTGTTCCGGCAATTGCAAT	CGTTCTGACCGACAACTCAT	45.0	50.0	58.2	57.9	567 bp	1.0	78.4
psaI-ycf4_p3	TTGTTCCGGCAATTGCAATG	CGTTCTGACCGACAACTCAT	45.0	50.0	58.2	57.9	566 bp	1.0	78.4
psaI-ycf4_p4	CTAGTTGTTCCGGCAATTGC	CGTTCTGACCGACAACTCAT	50.0	50.0	57.7	57.9	570 bp	1.0	77.9
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	TTGTTTTTCGAGACCCTGCT	45.0	45.0	57.9	57.7	597 bp	1.0	77.0