Hotspot

trnT-UGU-trnL-UAA

LSC · 48283-49377 · 1095 bp

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Pi 0.00183
MarkerSeek score 62.10
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1095
variable_sites
3
pi
0.00183
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CTCTAACCGCTGAGCTAAGC CGTACCAAGGCTCAATCCAA 55.0 50.0 58.2 57.9 1155-1176 bp 1.0 79.6
trnT-UGU-trnL-UAA_p2 CTCTAACCGCTGAGCTAAGC GTTTCCGTACCAAGGCTCAA 55.0 50.0 58.2 58.1 1160-1181 bp 1.0 79.5
trnT-UGU-trnL-UAA_p3 CTCTAACCGCTGAGCTAAGC CCGTAGCGTCTACCAATTCC 55.0 55.0 58.2 58.2 1129-1150 bp 1.0 79.2
trnT-UGU-trnL-UAA_p4 CTCTAACCGCTGAGCTAAGC AAGTTTCCGTACCAAGGCTC 55.0 50.0 58.2 57.8 1162-1183 bp 1.0 79.0
trnT-UGU-trnL-UAA_p5 CTCTAACCGCTGAGCTAAGC AGTCCGTAGCGTCTACCAAT 55.0 50.0 58.2 58.2 1132-1153 bp 1.0 78.5