Hotspot

rpoB-trnC-GCA

LSC · 26917-27889 · 973 bp

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Pi 0.00411

MarkerSeek score 61.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 6
pi: 0.00411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCCCTCATTTCCATCCCC	GCTTTTGTTGATCAGGCGAC	55.0	50.0	57.9	58.0	1083 bp	1.0	79.6
rpoB-trnC-GCA_p2	GTTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1061 bp	1.0	79.2
rpoB-trnC-GCA_p3	GTTCCCTCATTTCCATCCCC	AGCTTTTGTTGATCAGGCGA	55.0	45.0	57.9	58.1	1084 bp	1.0	79.0
rpoB-trnC-GCA_p4	CGTTCCCTCATTTCCATCCC	GCTTTTGTTGATCAGGCGAC	55.0	50.0	58.3	58.0	1084 bp	1.0	77.6
rpoB-trnC-GCA_p5	CGTTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1062 bp	1.0	77.2