Hotspot

psbK-psbI

LSC · 6690-7152 · 463 bp

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Pi 0.00576

MarkerSeek score 37.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 4
pi: 0.00576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCTGCTGTCAGTTTTCG	CCGAAGATGAAGAGCGAAAC	50.0	50.0	58.0	56.9	552 bp	1.0	71.9
psbK-psbI_p2	AAGCTGCTGTCAGTTTTCGA	CCGAAGATGAAGAGCGAAAC	45.0	50.0	58.0	56.9	551 bp	1.0	71.8
psbK-psbI_p3	AGCTGCTGTCAGTTTTCGAT	CCGAAGATGAAGAGCGAAAC	45.0	50.0	57.8	56.9	550 bp	1.0	70.7
psbK-psbI_p4	GCAAGCTGCTGTCAGTTTTC	CCGAAGATGAAGAGCGAAAC	50.0	50.0	58.2	56.9	553 bp	1.0	70.2
psbK-psbI_p5	CAGTCATCCCTGTGCTCTTT	CCGAAGATGAAGAGCGAAAC	50.0	50.0	57.5	56.9	593 bp	1.0	68.2