Hotspot

trnK-UUU-rps16

LSC · 4191-4767 · 577 bp

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Pi 0.00578

MarkerSeek score 51.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 5
pi: 0.00578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGCGATCTTCCCAACTCTAC	ACACAAAAAGGGGGTTGGAT	55.0	45.0	58.2	57.5	713-729 bp	1.0	75.4
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	ACACAAAAAGGGGGTTGGAT	50.0	45.0	58.2	57.5	633-649 bp	1.0	75.4
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	ACACAAAAAGGGGGTTGGAT	50.0	45.0	58.2	57.5	632-648 bp	1.0	75.4
trnK-UUU-rps16_p4	TCGCGATCTTCCCAACTCTA	ACACAAAAAGGGGGTTGGAT	50.0	45.0	58.2	57.5	714-730 bp	1.0	74.7
trnK-UUU-rps16_p5	TCTACAGATAGCGTGGACGA	ACACAAAAAGGGGGTTGGAT	50.0	45.0	57.7	57.5	698-714 bp	1.0	74.1