Hotspot

ccsA-ndhD

SSC · 114948-115158 · 211 bp

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Pi 0.0572

MarkerSeek score 80.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 211
variable_sites: 25
pi: 0.0572
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGTTTACATAGTTATGGTTCA	AGCAAGTGACAAGGTCGAAT	31.8	45.0	52.5	57.4	297-320 bp	1.0	49.7
ccsA-ndhD_p2	AGGTTTACATAGTTATGGTTCA	GCAAGTGACAAGGTCGAATC	31.8	50.0	52.5	57.1	296-319 bp	1.0	49.7
ccsA-ndhD_p3	AGGTTTACATAGTTATGGTTCAT	AGCAAGTGACAAGGTCGAAT	30.4	45.0	53.2	57.4	297-320 bp	1.0	49.7
ccsA-ndhD_p4	AGGAATAGGTTTACATAGTTATGGT	AGCAAGTGACAAGGTCGAAT	32.0	45.0	55.3	57.4	303-326 bp	1.0	49.5
ccsA-ndhD_p5	GGAATAGGTTTACATAGTTATGGTTCA	AGCAAGTGACAAGGTCGAAT	33.3	45.0	57.2	57.4	302-325 bp	1.0	49.5