Hotspot

rpl32-trnL-UAG

SSC · 113221-113771 · 551 bp

Back to Matthiola

Pi 0.0736
MarkerSeek score 82.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
551
variable_sites
75
pi
0.0736
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAGCACTCTTGTACGGCTTT TGGAAAAAGCCCACGAAAGA 50.0 45.0 58.2 57.9 1388-1392 bp 0.6 64.9
rpl32-trnL-UAG_p2 GAGCACTCTTGTACGGCTTT TAACCACTGGGACTGGAGAG 50.0 55.0 58.2 58.1 1549-1553 bp 0.6 64.9
rpl32-trnL-UAG_p3 AGCACTCTTGTACGGCTTTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 982-984 bp 0.4 56.4
rpl32-trnL-UAG_p4 AGCACTCTTGTACGGCTTTC TGGAAAAAGCCCACGAAAGA 50.0 45.0 58.2 57.9 1387-1391 bp 0.4 56.3
rpl32-trnL-UAG_p5 AGCACTCTTGTACGGCTTTC TAACCACTGGGACTGGAGAG 50.0 55.0 58.2 58.1 1548-1552 bp 0.4 56.3