Hotspot

ndhF-rpl32

SSC · 112179-113061 · 883 bp

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Pi 0.0621

MarkerSeek score 83.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 883
variable_sites: 96
pi: 0.0621
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTCCACATACGCCGAAGATT	TTTCACCATAGCGGCAACTT	50.0	45.0	58.1	58.1	1544-1731 bp	1.0	90.7
ndhF-rpl32_p2	GTCCACATACGCCGAAGATT	AGCGGCAACTTGTTCAAAAC	50.0	45.0	58.1	58.1	1535-1722 bp	0.8	82.9
ndhF-rpl32_p3	GGAAGCGGAATGAAAGGGAT	TTTCACCATAGCGGCAACTT	50.0	45.0	57.9	58.1	1476-1659 bp	0.6	70.0
ndhF-rpl32_p4	GGAAGCGGAATGAAAGGGAT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1500-1683 bp	0.6	69.8
ndhF-rpl32_p5	GGAAGCGGAATGAAAGGGAT	AGCGGCAACTTGTTCAAAAC	50.0	45.0	57.9	58.1	1467-1650 bp	0.4	60.9