Hotspot

petA-psbJ

LSC · 62085-63125 · 1041 bp

Back to Matthiola

Pi 0.0668

MarkerSeek score 80.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1041
variable_sites: 120
pi: 0.0668
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGAGGGCGAATCCATTAAA	TGGACTAGCTGTACCTACCG	50.0	55.0	58.0	58.0	1497-1724 bp	1.0	90.2
petA-psbJ_p2	CGGAGGGCGAATCCATTAAA	GGACTAGCTGTACCTACCGT	50.0	55.0	58.0	58.0	1496-1723 bp	1.0	90.2
petA-psbJ_p3	CGGAGGGCGAATCCATTAAA	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1614-1841 bp	1.0	90.1
petA-psbJ_p4	CGGAGGGCGAATCCATTAAA	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1613-1840 bp	1.0	90.1
petA-psbJ_p5	ATCCACTACGTGTTCAAGGC	ACAGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	1635-1747 bp	0.6	70.2