Hotspot

trnF-GAA-ndhJ

LSC · 47594-48124 · 531 bp

Back to Matthiola

Pi 0.0431

MarkerSeek score 81.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 42
pi: 0.0431
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATAGCCGGGATAGCTCAGT	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.0	58.0	677-748 bp	1.0	88.3
trnF-GAA-ndhJ_p2	AATAGCCGGGATAGCTCAGT	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.0	58.0	676-747 bp	1.0	88.3
trnF-GAA-ndhJ_p3	ATAGCCGGGATAGCTCAGTT	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.0	58.0	676-747 bp	1.0	88.3
trnF-GAA-ndhJ_p4	ATAGCCGGGATAGCTCAGTT	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.0	58.0	675-746 bp	1.0	88.3
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	633-704 bp	1.0	88.3