Hotspot

trnT-UGU-trnL-UAA

LSC · 45718-46549 · 832 bp

Back to Matthiola

Pi 0.0449

MarkerSeek score 83.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 71
pi: 0.0449
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CTACCGATTCCGCCATATCC	55.0	55.0	57.9	57.7	833-911 bp	1.0	87.0
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CTACCGATTCCGCCATATCC	50.0	55.0	57.8	57.7	865-943 bp	1.0	85.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	874-952 bp	1.0	85.1
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCGG	CTACCGATTCCGCCATATCC	55.0	55.0	57.5	57.7	825-903 bp	1.0	84.2
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	GGTTTCCATACCAAGGCTCA	50.0	50.0	57.8	57.5	906-984 bp	1.0	84.0