Hotspot

petN-psbM

LSC · 27945-28474 · 530 bp

Back to Matthiola

Pi 0.0492

MarkerSeek score 82.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 50
pi: 0.0492
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	AGTTCGAATCCAACCAAGGA	50.0	45.0	58.0	56.8	1249-1312 bp	0.6	62.4
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	AGTTCGAATCCAACCAAGGA	55.0	45.0	57.9	56.8	1248-1311 bp	0.6	61.8
petN-psbM_p3	CTCTCGTAGTGTGGGGAAGA	AGTTCGAATCCAACCAAGGA	55.0	45.0	58.2	56.8	1252-1315 bp	0.6	61.1
petN-psbM_p4	CTCTCTCGTAGTGTGGGGAA	AGTTCGAATCCAACCAAGGA	55.0	45.0	58.2	56.8	1254-1317 bp	0.6	61.1
petN-psbM_p5	TCTCTCGTAGTGTGGGGAAG	AGTTCGAATCCAACCAAGGA	55.0	45.0	58.2	56.8	1253-1316 bp	0.6	61.1