Hotspot

trnS-GCU-trnG-GGA

LSC · 7771-8427 · 657 bp

Back to Matthiola

Pi 0.06

MarkerSeek score 84.70

Primer available yes

Label trnS-GCU-trnG-GGA

Feature Metadata

label_name: trnS-GCU-trnG-GGA
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 74
pi: 0.06
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GGA_p1	CCAACGCTTTAGTCCACTCA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	719-819 bp	1.0	62.2
trnS-GCU-trnG-GGA_p2	AACGCTTTAGTCCACTCAGC	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	717-817 bp	1.0	61.9
trnS-GCU-trnG-GGA_p3	CCAACGCTTTAGTCCACTCA	AACGAATCACACTTTTACCACT	50.0	36.4	57.8	56.2	720-820 bp	1.0	60.3
trnS-GCU-trnG-GGA_p4	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	714-814 bp	1.0	60.3
trnS-GCU-trnG-GGA_p5	AACGCTTTAGTCCACTCAGC	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	718-818 bp	1.0	60.0