Hotspot

ycf1

SSC · 121860-127406 · 5547 bp

Back to Matsumurella

Pi 0.00649

MarkerSeek score 42.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 71
pi: 0.00649
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGATTTACTTTTGAAAGTTCTT	AACTCCCCTTTTGTTCACCC	26.1	50.0	52.7	57.9	227 bp	1.0	42.5
ycf1_p2	TTGGATTTACTTTTGAAAGTTCT	AACTCCCCTTTTGTTCACCC	26.1	50.0	52.7	57.9	228 bp	1.0	42.5
ycf1_p3	TGGATTTACTTTTGAAAGTTCTTT	AACTCCCCTTTTGTTCACCC	25.0	50.0	53.6	57.9	227 bp	1.0	42.5
ycf1_p4	TTGGATTTACTTTTGAAAGTTCTT	AACTCCCCTTTTGTTCACCC	25.0	50.0	53.6	57.9	228 bp	1.0	42.5
ycf1_p5	AGAATTGGATTTACTTTTGAAAGTTCT	AACTCCCCTTTTGTTCACCC	25.9	50.0	56.4	57.9	232 bp	1.0	42.4