Hotspot

rbcL

LSC · 54588-56054 · 1467 bp

Back to Matsumurella

Pi 0.00491

MarkerSeek score 54.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 13
pi: 0.00491
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AACGCCCAATTCATTCGTGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	58.8	58.4	1566-1575 bp	1.0	71.6
rbcL_p2	AACGCCCAATTCATTCGTGT	TTGGGCCGAGTTTAATTGCA	45.0	45.0	58.8	58.4	1567-1576 bp	1.0	71.6
rbcL_p3	GCCCAATTCATTCGTGTCGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	58.9	58.4	1563-1572 bp	1.0	70.3
rbcL_p4	GCCCAATTCATTCGTGTCGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	58.9	58.4	1564-1573 bp	1.0	70.3
rbcL_p5	ACGCCCAATTCATTCGTGT	TGGGCCGAGTTTAATTGCAA	47.4	45.0	58.1	58.4	1565-1574 bp	1.0	69.2