Hotspot

petD

LSC · 75527-76748 · 1222 bp

Back to Matsumurella

Pi 0.00627

MarkerSeek score 43.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1222
variable_sites: 13
pi: 0.00627
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCCGA	GTCGTGTTATTTCACAATTGAATCA	45.0	32.0	56.2	56.9	1274-1283 bp	1.0	41.4
petD_p2	TGAAGGGAATTCTCCGAAGAG	GTCGTGTTATTTCACAATTGAATCA	47.6	32.0	57.1	56.9	1271-1280 bp	1.0	41.4
petD_p3	AGTTGAAGGGAATTCTCCGA	GTCGTGTTATTTCACAATTGA	45.0	33.3	56.2	52.8	1274-1283 bp	1.0	41.4
petD_p4	TGAAGGGAATTCTCCGAAGAG	GTCGTGTTATTTCACAATTGA	47.6	33.3	57.1	52.8	1271-1280 bp	1.0	41.4
petD_p5	AGTTGAAGGGAATTCTCCGAAG	GTCGTGTTATTTCACAATTGAATCA	45.5	32.0	58.1	56.9	1274-1283 bp	1.0	41.4