Hotspot

ndhF

SSC · 108665-110887 · 2223 bp

Back to Matsumurella

Pi 0.00648

MarkerSeek score 41.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 28
pi: 0.00648
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AATCCGGGAAAATATGCCCC	50.0	50.0	58.0	58.0	2737-2738 bp	1.0	80.6
ndhF_p2	AAGAGGGATCCACCGAAGAA	AATCCGGGAAAATATGCCCC	50.0	50.0	58.0	58.0	2729-2730 bp	1.0	80.6
ndhF_p3	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2519-2520 bp	1.0	80.5
ndhF_p4	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2518-2519 bp	1.0	80.5
ndhF_p5	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2510-2511 bp	1.0	80.5