Hotspot

ndhA

SSC · 117758-119923 · 2166 bp

Back to Matsumurella

Pi 0.00665

MarkerSeek score 56.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2166
variable_sites: 29
pi: 0.00665
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	42.9	50.0	56.1	58.0	2384-2396 bp	1.0	57.7
ndhA_p2	GAACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	43.5	50.0	57.9	58.0	2386-2398 bp	1.0	56.4
ndhA_p3	ACTGAGTTAGCATAGGGAACA	CGCCGGGTTTTATCAACTTG	42.9	50.0	56.1	57.7	2385-2397 bp	1.0	55.7
ndhA_p4	AACTGAGTTAGCATAGGGAACA	GCCGGGTTTTATCAACTTGC	40.9	50.0	56.8	58.0	2385-2397 bp	1.0	55.7
ndhA_p5	GAACTGAGTTAGCATAGGGAACA	CGCCGGGTTTTATCAACTTG	43.5	50.0	57.9	57.7	2387-2399 bp	1.0	54.4